LAMP TechnologyLoop-mediated isothermal amplification
The LAMP method is a simple and rapid isothermal amplification method using Bst DNA polymerase with strand displacement DNA synthesis function. Two pairs of specific primers are used to form a loop and amplify continuously.
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Fig.1
Generate LAMP primer matching sequence listing in target DNA.
Each primer site is designated as forward
5'- (F3, F2, F1, B1c, B2c, B3c) -3`, Revers 3`- (F3c, F2c, F1c, B1.
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Fig.2
Primer design for sequence listing in target DNA
[forward outer primer F3, forward inner primer FIP(F1c + F2),
Backward outer primer B3, Backward inner primer BIP(B1c, B2)] -
Fig.3
DNA amplification after binding FIP primer
with target DNA.
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Fig.5
DNA amplification by F3 primer binding.,
amplification by FIP. Isolation of single-strand DNA
from double-strand DNA - Fig.4
After DNA amplification by FIP primer,
F3 primer combination and DNA amplification
- Fig.6
After BIP primer binding to single-strand DNA
which is isolated by F3 primer binding, DNA amplification.
Formation of double-strand DNA - Fig.7
B3 primer binding
to the formed double-strand DNA
- Fig.9
DNA formation with sequence listing of
3`- F1, F2c, F1C B1, B2, B1c -5` from target DNA sequence
listing reverses 3'- (F3c, F2c, F1c, B1, B2, B3) -5`. At this time,
sequence listing F1c complementary to F1, B1c complementarym
to B1 binds to form loop-shaped DNA. - Fig.8
Single-strand DNA isolation
from double-strand DNA by B3 primer binding
Then, DNA amplify sequentially by combining loop formed DNA
as designed primers F3, FIP (F1c + F2), B3 and BIP.
