메뉴바로가기

본문 내용으로 바로가기 메뉴으로 바로가기

More accurate & Faster diagnosis.
M-monitor Inc. research products
for human health and welfare.

LAMP TechnologyLoop-mediated isothermal amplification

The LAMP method is a simple and rapid isothermal amplification method using Bst DNA polymerase with strand displacement DNA synthesis function. Two pairs of specific primers are used to form a loop and amplify continuously.

  • Fig.1

    Generate LAMP primer matching sequence listing in target DNA.
    Each primer site is designated as forward
    5'- (F3, F2, F1, B1c, B2c, B3c) -3`, Revers 3`- (F3c, F2c, F1c, B1.

  • Fig.2

    Primer design for sequence listing in target DNA
    [forward outer primer F3, forward inner primer FIP(F1c + F2),
    Backward outer primer B3, Backward inner primer BIP(B1c, B2)]

  • Fig.3

    DNA amplification after binding FIP primer
    with target DNA.

  • Fig.5

    DNA amplification by F3 primer binding.,
    amplification by FIP. Isolation of single-strand DNA
    from double-strand DNA

  • Fig.4

    After DNA amplification by FIP primer,
    F3 primer combination and DNA amplification

  • Fig.6

    After BIP primer binding to single-strand DNA
    which is isolated by F3 primer binding, DNA amplification.
    Formation of double-strand DNA

  • Fig.7

    B3 primer binding
    to the formed double-strand DNA

  • Fig.9

    DNA formation with sequence listing of
    3`- F1, F2c, F1C B1, B2, B1c -5` from target DNA sequence
    listing reverses 3'- (F3c, F2c, F1c, B1, B2, B3) -5`. At this time,
    sequence listing F1c complementary to F1, B1c complementarym
    to B1 binds to form loop-shaped DNA.

  • Fig.8

    Single-strand DNA isolation
    from double-strand DNA by B3 primer binding

  • Then, DNA amplify sequentially by combining loop formed DNA
    as designed primers F3, FIP (F1c + F2), B3 and BIP.